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Blockade of adipose sympathetic nerve does not prevent chronic stress-induced impairment of hepatic lipid homeostasis. (A-D) FFA, leptin, adiponectin, resistin levels in serum, n = 10 per group. (E) Experimental scheme. (F) Body weight, n= 5:6. (G) Food intake, n = 5:6. (H-K) Serum ALT, AST, TG and TC concentrations, n = 5:6. (L-O) FFA, leptin, adiponectin, resistin levels in serum, n = 5:6. (P) Liver weight and liver anatomy, n = 5:6. (Q) Liver TG, n = 5:6. (R) H&E staining (scale bars, 20 μm) and Oil Red O staining (scale bars, 20 μm) of liver sections, n = 5:6. (S) Relative mRNA levels in liver, n = 5:6. The data are presented as mean ± SEM. * P < 0.05. Unpaired two-tailed Student's t -test and one-way ANOVA were used for statistical analysis.

Journal: International Journal of Biological Sciences

Article Title: Chronic Stress Induces Hepatic Steatosis via Brain-Hepatic Sympathetic Axis Mediated Catecholamine Resistance

doi: 10.7150/ijbs.126058

Figure Lengend Snippet: Blockade of adipose sympathetic nerve does not prevent chronic stress-induced impairment of hepatic lipid homeostasis. (A-D) FFA, leptin, adiponectin, resistin levels in serum, n = 10 per group. (E) Experimental scheme. (F) Body weight, n= 5:6. (G) Food intake, n = 5:6. (H-K) Serum ALT, AST, TG and TC concentrations, n = 5:6. (L-O) FFA, leptin, adiponectin, resistin levels in serum, n = 5:6. (P) Liver weight and liver anatomy, n = 5:6. (Q) Liver TG, n = 5:6. (R) H&E staining (scale bars, 20 μm) and Oil Red O staining (scale bars, 20 μm) of liver sections, n = 5:6. (S) Relative mRNA levels in liver, n = 5:6. The data are presented as mean ± SEM. * P < 0.05. Unpaired two-tailed Student's t -test and one-way ANOVA were used for statistical analysis.

Article Snippet: Serum leptin (E-EL-M3008), adiponectin (E-EL-M0002), resistin (E-EL-M3056), norepinephrine (NE, E-EL-0047), and hepatic cyclic adenosine monophosphate (cAMP, E-EL-0056) in liver extracts were measured using enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer's intructions (Elabscience, Wuhan, China).

Techniques: Staining, Two Tailed Test